動態(tài)超高壓微射流對卵清蛋白微觀結構的影響

摘要：采用圓二色譜(CD)、X射線衍射(XRD)、ANS熒光探針和紫外(UV)光譜研究了動態(tài)超高壓微射流對卵清蛋白微觀結構的影響.結果表明:卵清蛋白的微觀結構的變化與處理壓力有關,CD顯示不同壓力處理的卵清蛋白二級結構中的α-螺旋,β-折疊,β-轉角和無規(guī)卷曲之間發(fā)生相互轉化,二級結構的有序性提高;X射線衍射圖譜直觀顯示不同壓力處理的卵清蛋白品體結構增加,160 MPa處理下結晶區(qū)最大,說明蛋白結構的有序性提高,與CD分析結果相似;ANS熒光探針光譜顯示卵清蛋白的表面疏水性隨著處理壓力的`增大而提高,120 MPa處理下達到最大;紫外光譜顯示隨著處理壓力的增大,卵清蛋白最大紫外吸光值下降,卵清蛋白分子表面的具有紫外吸收的芳香氨基酸殘基被包埋于分子內部,卵清蛋白的三維結構發(fā)生改變.Abstract：The effect of dynamic high pressure microfluidization on the microstructure of ovalbumin was studied by CD spectra,XRD spectra,ANS fluorescence probe emission spectra and UV absorption spectra.The results indicated that the changes in the microstructure were dependent on the pressure.CD spectra were used to examine the changes in the secondary structure of the ovalbumin treated by different pressures.When the pressure increased,the mutual transformation between α-helix,β-sheet,β-tum and the random coil was observed.The orderliness of the secondary structure was increased with increasing the pressure.XRD spectra analysis showed that the crystal structure content of the ovalbumin treated increased with increasing the pressure and the largest data was observed at 160 MPa,indicating that the orderliness of the secondary structure was increased.The results were similar to CD spectra analysis.The ANS fluorescence probe emission spectra analysis demonstrated that the dynamic high pressure microfluidization induced an increase in surface hydrophobicity following high pressure treatment,while the largest data was observed at 120 MPa.In addition,UV absorption spectra analysis indicated that dynamic high pressure microfluidization treatment also resulted in a decrease in the UV-absorption maximum wavelength with increasing the pressure,indicating that the aromatic amino acid was buried in the molecular interior and the three dimensional structure of ovalbumin was changed. 作者： 涂宗財王輝劉光憲陳鋼豆玉新張雪春 Author： TU Zong-cai  WANG Hui  LIU Guang-xian  CHEN Guang  DOU Yu-xin  ZHANG Xue-chun 作者單位： 南昌大學食品科學與技術國家重點實驗室,江西,南昌,330047 期 刊： 光譜學與光譜分析   ISTICEISCIPKU Journal： SPECTROSCOPY AND SPECTRAL ANALYSIS 年，卷(期)： 2010, 30(2) 分類號： Q0631.1 關鍵詞： 動態(tài)超高壓微射流    卵清蛋白    圓二色譜    X射線衍射    ANS熒光探針    紫外光譜    Keywords： Dynamic high pressure microfluidization    Ovalbumin    Circular dichroism    XRD spectra    ANS fluorescence probe    Ultraviolet spectra    機標分類號： R91 R9 機標關鍵詞： 動態(tài)    超高壓微射流    卵清蛋白    微觀結構    Microstructure    Treatment    High Pressure    absorption spectra    secondary structure    high pressure    fluorescence probe    emission spectra    analysis    壓力處理    microstructure    UV absorption    ANS熒光探針    dynamic    aromatic amino acid    treatment 基金項目： 國家自然科學基金，教育部長江學者獎勵計劃

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